This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Vasquez, R. Articles by Raffetto, J. D. Search for Related Content PubMed PubMed Citation Articles by Vasquez, R. Articles by Raffetto, J. D. Social Bookmarking                         What's this?

Proliferative Capacity of Venous Ulcer Wound Fibroblasts in the Presence of Platelet-Derived Growth Factor

Brian J. Marien, MD

Christopher Gram, MD

David G. Goodwin, BS

James O. Menzoian, MD

Joseph D. Raffetto, MD

Boston, MA and Wilkes-Barre, PA

Growth factors have been demonstrated to increase the proliferation of wound fibroblasts. Platelet-derived growth factor (PDGF) is a potent cell mitogen. However, the role of PDGF in chronic venous ulcers is inconclusive. This study investigated whether PDGF stimulates venous ulcer fibroblasts to proliferate. Fibroblasts (fb) were isolated from 8 venous ulcers wounds (w-fb) and normal skin (n-fb) of the ipsilateral thigh via punch biopsies. Fibroblasts were plated at 1,500 cells/dish in Dulbecco's Modified Eagle Medium + 10% calf serum (CM) and treated with/without PDGF-ab (10 ng/mL) for 15 days. Growth rates were calculated. Western blotting and immunocytochemistry staining determined basal levels for PDGF-a and-b receptors, respectively. Growth rates were significantly lower in w-fb than in n-fb (1,579 ±546 vs 13,782 ±5,882 cells/day, p = 0.019). PDGF-ab treatment caused n-fb to increase their proliferative capacity relative to complete media (20,393 ±6,572 vs 13,782 ±5,882 cells/day, p= 0.005). However, PDGF-ab had no significant effect on w-fb proliferation over CM (1,030 ±264 and 1,579 ±546 cells/day, p=0.15). In the presence of PDGF-ab, w-fb had a significantly attenuated growth rate over n-fb (1,030 ±264 vs 20,393 ±6,572 cells/day, p=0.019). Western blot and immunocytochemistry analysis revealed diminished basal levels of PDGF-a and-b receptors, respectively, in ulcer fibroblasts. Venous ulcer fibroblasts had decreased proliferation. PDGF-ab had no effect on the growth rate of venous ulcer fibroblasts. In venous ulcers, decreased basal levels of fibroblast PDGF-a and-b receptors may explain reduced proliferation. Further clinical studies are needed to elucidate the role growth factors may play in venous ulcers.

Vascular and Endovascular Surgery, Vol. 38, No. 4, 355-360 (2004)
DOI: 10.1177/153857440403800408


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. D. Raffetto, C. H. Gram, K. C. Overman, and J. O. Menzoian Mitogen-Activated Protein Kinase p38 Pathway in Venous Ulcer Fibroblasts Vascular and Endovascular Surgery, August 1, 2008; 42(4): 367 - 374. [Abstract] [PDF]

				N. I. Chaudhary, A. Schnapp, and J. E. Park Pharmacologic Differentiation of Inflammation and Fibrosis in the Rat Bleomycin Model Am. J. Respir. Crit. Care Med., April 1, 2006; 173(7): 769 - 776. [Abstract] [Full Text] [PDF]