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The Effects of Glucose-Insulin-Potassium Solution and BN 52021 in Intestinal Ischemia-Reperfusion Injury

Department of General Surgery, Dicle University, Faculty of Medicine, Turkey; Dicle Universitesi, Tip Fakültesi, ILK ve ACIL YARDIM ABD, 21280, Diyarbakir, Turkeymaldemir21{at}hotmail.com

Ahmet Gürel, MD

Department of Biochemistry, Zonguldak Karaelmas University, Faculty of Medicine, Diyarbakir, Turkey

Hüseyin Büyükbayram, MD

Department of Pathology, Dicle University, Faculty of Medicine, Diyarbakir, Turkey

Ibrahim TaÁyildiz, MD

Department of General Surgery, Dicle University, Faculty of Medicine, Diyarbakir, Turkey

The objective of this study was to investigate effects of glucose-insulin-potassium (GIK) solution and BN 52021, a platelet-activating factor antagonist, on intestinal ischemiareperfusion injury. Fifty male Sprague-Dawley rats (200–225 g) were divided into 5 groups each containing 10 rats; group SO, sham operation group; group I, mesenteric ischemia group (for 30 minutes); group R, ischemia plus reperfusion (for 60 minutes); group BR, ischemia-reperfusion plus BN 52021; group GR, ischemia-reperfusion plus GIK solution. Samples for malondialdehyde (MDA) and ileum (for mucosal injury score) were obtained. The mucosal injury scores of group R were significantly higher than those of group I (4 ±0.20 and 3 ±0.16, respectively, p<0.0001). The scores of groups BR and GR were significantly lower than those of group R (p<0.0001 and p<0.0001, respectively). When it was compared with the injuries in BR and GR groups, similar results were obtained in both groups (p=0.190). Mean MDA levels of group R were significantly higher than those of group I, BR and GR (131.33 ±3.99 nmol/g, 93.74 ±3.22 nmol/g, 104.81 ±2.56 and 100.34 ±5.30, respectively, p<0.0001). MDA levels of group BR and GR were significantly lower than those of group I (p<0.0001 and p=0.003, respectively). These observations suggest that treatment with GIK solution and BN 52021 before reperfusion and during reperfusion period may be useful in decreasing intestinal reperfusion injury.

Vascular and Endovascular Surgery, Vol. 37, No. 5, 345-351 (2003)
DOI: 10.1177/153857440303700506


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