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Lipid Peroxides in the Mechanism of Ischemia/Reperfusion Injury in Skeletal Muscle—Experimental Studies

Nagoya University School of Medicine, Nagoya, Japan

N. Nishikimi

Nagoya University School of Medicine, Nagoya, Japan

Y. Oba

Nagoya University School of Medicine, Nagoya, Japan

Two sets of experiments were conducted to investigate whether oxygen-de rived free radicals are involved in the mechanism of ischemia/reperfusion (I/R) injury of skeletal muscle and vitamin E is effective in alleviating the injury.

In the first set of experiments, twenty adult mongrel dogs were divided into three groups: group 1 (n=7) control, group 2 (n = 6) I/R, and group 3 (n=7) I/R with 500 mg of vitamin E. Serum creatine phosphokinase (CPK) and lipid per oxides (LPO) were measured as markers of rhabdomyolysis and biomembrane injury due to oxygen-derived free radicals, respectively. CPK increased signifi cantly after reperfusion with a peak value of 38,000 ± 9,800 mU/mL in group 2, when compared with a peak value of 1,060 ± 290 mU/mL in group 3 (p < 0.02). LPO also significantly increased with a peak value of 20.4 ±3.7 nmol/mL in group 2, when compared with a peak value of 9.2 ± 2.2 nmol/mL in group 3 (p < 0.04).

In the second sets of experiments, 13 dogs were divided into two groups: group A (n=5) control and group B (n=8) I/R. Tissue LPO was measured eighteen hours after reperfusion in the gracilis muscle and gastrocnemius mus cle. The mean value of LPO in the gracilis muscle was 0.94 ± 0.46 nmol/mg protein in group A, compared with 1.13 ± 0.67 nmol/mg protein in group B. It was higher in group B, but there was no significant difference. Mean LPO in the gastrocnemius muscle was 0.85 ± 0.34 nmol/mg protein in group A, and 1.83 ± 0.71 nmol/mg protein in group B. There was a significant difference (p < 0.02).

Serum CPK and LPO were significantly higher in group 2 than in groups 1 and 3, and tissue LPO was also high in skeletal muscle after reperfusion. Serum CPK and LPO were effectively suppressed by administration of vitamin E be fore reperfusion.

These results show that oxygen-derived free radicals are involved in the mechanism of I/R injury in skeletal muscle and suggest that vitamin E is effec tive in alleviating the injury.

Vascular and Endovascular Surgery, Vol. 27, No. 3, 191-201 (1993)
DOI: 10.1177/153857449302700306


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