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Vascular and Endovascular Surgery
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Methods for the Initiation and Maintenance of Human Endothelial Cell Culture

Patrick S. Vaccaro

Department of Surgery, The Ohio State University College of Medicine

Laurie B. Joseph

Department of Pathology, The Ohio State University College of Medicine, Columbus, Ohio

Lynda Titterington

Department of Pathology, The Ohio State University College of Medicine, Columbus, Ohio

Ralph E. Stephens

Department of Pathology, The Ohio State University College of Medicine, Columbus, Ohio

The culture of human endothelial cells for the in vitro investigation of the metabolic, synthetic, and physiologic functions of these cells has grown increas ingly common. Model systems investigating the pathogenesis of atherosclerosis are being developed. Methods for the initiation and maintenance of human en dothelial cell cultures are described. The roles of cryopreservation, microcar rier culturing, and roller bottle mass culturing are discussed.

Vascular and Endovascular Surgery, Vol. 21, No. 6, 391-400 (1987)
DOI: 10.1177/153857448702100604
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